Semester VI, Paper-VIII: Tissue Culture and Biotechnology -Practical Question Bank


GIRRAJ GOVT.COLLEGE (A), NIZAMABAD
B.Sc (CBCS) Botany-III Year
Semester-VI
Subject:Botany
Paper-VIII: Tissue Culture and Biotechnology

Time: 3 hrs                                                                                       Max. Marks: 50

Practical Question Bank

I. Major Experiment                                                                                  (1 x 15=15 marks)
     1. Estimation of DNA
     2. Production of synthetic seeds /Encapsulation of embryo
     3. Preparation of plant tissue culture medium
II. Minor Experiment                                                                                (1x 10=10 marks)
      4. Isolation of plant DNA. (Tomato)
      5. Callus Micropropagation
      6 Demonstration of Micro propagation/ multiple shoots
      7 Anther culture
      8. PCR –Demonstration
III. Spotters –C,D,E,F,G,H                                                                        (3x6=18) marks                                                                                                                 
        (Vaccines-2, Antibiotics-2, Transgeneric plants-1, Instruments-1 )  

      C, D- Vaccines -    Polio (IPV), Hepatitis B, Varicella(chickenpox)  
      E, F- Antibiotics-   Penicillin, streptomycin, amoxicillin
         G- Transgeneric plants- Bt-Cotton, Bt-Brinjal
        H - instruments- Autoclave, Laminar air flow, Hot air oven and Incubator.

IV. Viva                                                                                                         ( 3 marks)
V.Record                                                                                                      ( 4 marks)


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